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Diminished Expression Level of Cytoskeletal-Related Genes in Vascular Remodeling Lesions and Cell Transition Model. (A-B) Peri-adventitial elastase (E1250, Sigma, ≥4.0 units/mg protein) or heat-inactivated elastase exposure induced mouse aneurysm model samples using 8 weeks old male C57BL6 mice. Contractile genes and cytoskeletal related genes expression level were tested with realtime PCR (A) and western blot (B1) with quantitation (B2). N = 6 for each treatment group. (C) Immunofluorescence staining to test expression changes of FBLIM1 (C1), TNS1 (C2), and SYNPO2 (C3) changes comparing αSMA high expression regions to low expression regions (C3). αSMA was used for cell type indicator of VSMCs. Lu indicates the lumen of the abdominal aorta in (C). N = 6 for sections from different mice. (D, E) Samples <t>from</t> <t>PDGF-BB</t> <t>(HY-P7087,</t> MCE, 20 μg/L) promoted MASMC in vitro transition model. Contractile genes and cytoskeletal-related gene expression levels were quantified with real-time quantitative RT-PCR (D) and western blot (E1) with quantitation (E2). N = 6 for each treatment group. Scale bars are as labeled. For all panels, data are expressed as Mean ± SEM and data points indicated biological replication. Multiple unpaired t test with Welch correction was used two compare group-wise mean differences and two-stage step-up (Benjamini, Krieger, and Yekutieli) method to control False Discovery Rate (FDR) < 1 %. The p -values are annotated for indicated comparisons.
Pdgf Bb, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Diminished Expression Level of Cytoskeletal-Related Genes in Vascular Remodeling Lesions and Cell Transition Model. (A-B) Peri-adventitial elastase (E1250, Sigma, ≥4.0 units/mg protein) or heat-inactivated elastase exposure induced mouse aneurysm model samples using 8 weeks old male C57BL6 mice. Contractile genes and cytoskeletal related genes expression level were tested with realtime PCR (A) and western blot (B1) with quantitation (B2). N = 6 for each treatment group. (C) Immunofluorescence staining to test expression changes of FBLIM1 (C1), TNS1 (C2), and SYNPO2 (C3) changes comparing αSMA high expression regions to low expression regions (C3). αSMA was used for cell type indicator of VSMCs. Lu indicates the lumen of the abdominal aorta in (C). N = 6 for sections from different mice. (D, E) Samples <t>from</t> <t>PDGF-BB</t> <t>(HY-P7087,</t> MCE, 20 μg/L) promoted MASMC in vitro transition model. Contractile genes and cytoskeletal-related gene expression levels were quantified with real-time quantitative RT-PCR (D) and western blot (E1) with quantitation (E2). N = 6 for each treatment group. Scale bars are as labeled. For all panels, data are expressed as Mean ± SEM and data points indicated biological replication. Multiple unpaired t test with Welch correction was used two compare group-wise mean differences and two-stage step-up (Benjamini, Krieger, and Yekutieli) method to control False Discovery Rate (FDR) < 1 %. The p -values are annotated for indicated comparisons.
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Diminished Expression Level of Cytoskeletal-Related Genes in Vascular Remodeling Lesions and Cell Transition Model. (A-B) Peri-adventitial elastase (E1250, Sigma, ≥4.0 units/mg protein) or heat-inactivated elastase exposure induced mouse aneurysm model samples using 8 weeks old male C57BL6 mice. Contractile genes and cytoskeletal related genes expression level were tested with realtime PCR (A) and western blot (B1) with quantitation (B2). N = 6 for each treatment group. (C) Immunofluorescence staining to test expression changes of FBLIM1 (C1), TNS1 (C2), and SYNPO2 (C3) changes comparing αSMA high expression regions to low expression regions (C3). αSMA was used for cell type indicator of VSMCs. Lu indicates the lumen of the abdominal aorta in (C). N = 6 for sections from different mice. (D, E) Samples <t>from</t> <t>PDGF-BB</t> <t>(HY-P7087,</t> MCE, 20 μg/L) promoted MASMC in vitro transition model. Contractile genes and cytoskeletal-related gene expression levels were quantified with real-time quantitative RT-PCR (D) and western blot (E1) with quantitation (E2). N = 6 for each treatment group. Scale bars are as labeled. For all panels, data are expressed as Mean ± SEM and data points indicated biological replication. Multiple unpaired t test with Welch correction was used two compare group-wise mean differences and two-stage step-up (Benjamini, Krieger, and Yekutieli) method to control False Discovery Rate (FDR) < 1 %. The p -values are annotated for indicated comparisons.
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Diminished Expression Level of Cytoskeletal-Related Genes in Vascular Remodeling Lesions and Cell Transition Model. (A-B) Peri-adventitial elastase (E1250, Sigma, ≥4.0 units/mg protein) or heat-inactivated elastase exposure induced mouse aneurysm model samples using 8 weeks old male C57BL6 mice. Contractile genes and cytoskeletal related genes expression level were tested with realtime PCR (A) and western blot (B1) with quantitation (B2). N = 6 for each treatment group. (C) Immunofluorescence staining to test expression changes of FBLIM1 (C1), TNS1 (C2), and SYNPO2 (C3) changes comparing αSMA high expression regions to low expression regions (C3). αSMA was used for cell type indicator of VSMCs. Lu indicates the lumen of the abdominal aorta in (C). N = 6 for sections from different mice. (D, E) Samples <t>from</t> <t>PDGF-BB</t> <t>(HY-P7087,</t> MCE, 20 μg/L) promoted MASMC in vitro transition model. Contractile genes and cytoskeletal-related gene expression levels were quantified with real-time quantitative RT-PCR (D) and western blot (E1) with quantitation (E2). N = 6 for each treatment group. Scale bars are as labeled. For all panels, data are expressed as Mean ± SEM and data points indicated biological replication. Multiple unpaired t test with Welch correction was used two compare group-wise mean differences and two-stage step-up (Benjamini, Krieger, and Yekutieli) method to control False Discovery Rate (FDR) < 1 %. The p -values are annotated for indicated comparisons.
Pdgf, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Diminished Expression Level of Cytoskeletal-Related Genes in Vascular Remodeling Lesions and Cell Transition Model. (A-B) Peri-adventitial elastase (E1250, Sigma, ≥4.0 units/mg protein) or heat-inactivated elastase exposure induced mouse aneurysm model samples using 8 weeks old male C57BL6 mice. Contractile genes and cytoskeletal related genes expression level were tested with realtime PCR (A) and western blot (B1) with quantitation (B2). N = 6 for each treatment group. (C) Immunofluorescence staining to test expression changes of FBLIM1 (C1), TNS1 (C2), and SYNPO2 (C3) changes comparing αSMA high expression regions to low expression regions (C3). αSMA was used for cell type indicator of VSMCs. Lu indicates the lumen of the abdominal aorta in (C). N = 6 for sections from different mice. (D, E) Samples <t>from</t> <t>PDGF-BB</t> <t>(HY-P7087,</t> MCE, 20 μg/L) promoted MASMC in vitro transition model. Contractile genes and cytoskeletal-related gene expression levels were quantified with real-time quantitative RT-PCR (D) and western blot (E1) with quantitation (E2). N = 6 for each treatment group. Scale bars are as labeled. For all panels, data are expressed as Mean ± SEM and data points indicated biological replication. Multiple unpaired t test with Welch correction was used two compare group-wise mean differences and two-stage step-up (Benjamini, Krieger, and Yekutieli) method to control False Discovery Rate (FDR) < 1 %. The p -values are annotated for indicated comparisons.
Human Pdgf Bb His, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Diminished Expression Level of Cytoskeletal-Related Genes in Vascular Remodeling Lesions and Cell Transition Model. (A-B) Peri-adventitial elastase (E1250, Sigma, ≥4.0 units/mg protein) or heat-inactivated elastase exposure induced mouse aneurysm model samples using 8 weeks old male C57BL6 mice. Contractile genes and cytoskeletal related genes expression level were tested with realtime PCR (A) and western blot (B1) with quantitation (B2). N = 6 for each treatment group. (C) Immunofluorescence staining to test expression changes of FBLIM1 (C1), TNS1 (C2), and SYNPO2 (C3) changes comparing αSMA high expression regions to low expression regions (C3). αSMA was used for cell type indicator of VSMCs. Lu indicates the lumen of the abdominal aorta in (C). N = 6 for sections from different mice. (D, E) Samples <t>from</t> <t>PDGF-BB</t> <t>(HY-P7087,</t> MCE, 20 μg/L) promoted MASMC in vitro transition model. Contractile genes and cytoskeletal-related gene expression levels were quantified with real-time quantitative RT-PCR (D) and western blot (E1) with quantitation (E2). N = 6 for each treatment group. Scale bars are as labeled. For all panels, data are expressed as Mean ± SEM and data points indicated biological replication. Multiple unpaired t test with Welch correction was used two compare group-wise mean differences and two-stage step-up (Benjamini, Krieger, and Yekutieli) method to control False Discovery Rate (FDR) < 1 %. The p -values are annotated for indicated comparisons.
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Diminished Expression Level of Cytoskeletal-Related Genes in Vascular Remodeling Lesions and Cell Transition Model. (A-B) Peri-adventitial elastase (E1250, Sigma, ≥4.0 units/mg protein) or heat-inactivated elastase exposure induced mouse aneurysm model samples using 8 weeks old male C57BL6 mice. Contractile genes and cytoskeletal related genes expression level were tested with realtime PCR (A) and western blot (B1) with quantitation (B2). N = 6 for each treatment group. (C) Immunofluorescence staining to test expression changes of FBLIM1 (C1), TNS1 (C2), and SYNPO2 (C3) changes comparing αSMA high expression regions to low expression regions (C3). αSMA was used for cell type indicator of VSMCs. Lu indicates the lumen of the abdominal aorta in (C). N = 6 for sections from different mice. (D, E) Samples from PDGF-BB (HY-P7087, MCE, 20 μg/L) promoted MASMC in vitro transition model. Contractile genes and cytoskeletal-related gene expression levels were quantified with real-time quantitative RT-PCR (D) and western blot (E1) with quantitation (E2). N = 6 for each treatment group. Scale bars are as labeled. For all panels, data are expressed as Mean ± SEM and data points indicated biological replication. Multiple unpaired t test with Welch correction was used two compare group-wise mean differences and two-stage step-up (Benjamini, Krieger, and Yekutieli) method to control False Discovery Rate (FDR) < 1 %. The p -values are annotated for indicated comparisons.

Journal: Journal of Advanced Research

Article Title: Cytoskeletal-related genes function as checkpoints for the maintenance of VSMC contractile phenotype and prevent pathological remodeling in arterial diseases

doi: 10.1016/j.jare.2025.05.065

Figure Lengend Snippet: Diminished Expression Level of Cytoskeletal-Related Genes in Vascular Remodeling Lesions and Cell Transition Model. (A-B) Peri-adventitial elastase (E1250, Sigma, ≥4.0 units/mg protein) or heat-inactivated elastase exposure induced mouse aneurysm model samples using 8 weeks old male C57BL6 mice. Contractile genes and cytoskeletal related genes expression level were tested with realtime PCR (A) and western blot (B1) with quantitation (B2). N = 6 for each treatment group. (C) Immunofluorescence staining to test expression changes of FBLIM1 (C1), TNS1 (C2), and SYNPO2 (C3) changes comparing αSMA high expression regions to low expression regions (C3). αSMA was used for cell type indicator of VSMCs. Lu indicates the lumen of the abdominal aorta in (C). N = 6 for sections from different mice. (D, E) Samples from PDGF-BB (HY-P7087, MCE, 20 μg/L) promoted MASMC in vitro transition model. Contractile genes and cytoskeletal-related gene expression levels were quantified with real-time quantitative RT-PCR (D) and western blot (E1) with quantitation (E2). N = 6 for each treatment group. Scale bars are as labeled. For all panels, data are expressed as Mean ± SEM and data points indicated biological replication. Multiple unpaired t test with Welch correction was used two compare group-wise mean differences and two-stage step-up (Benjamini, Krieger, and Yekutieli) method to control False Discovery Rate (FDR) < 1 %. The p -values are annotated for indicated comparisons.

Article Snippet: N = 6 for sections from different mice. (D, E) Samples from PDGF-BB (HY-P7087, MCE, 20 μg/L) promoted MASMC in vitro transition model. Contractile genes and cytoskeletal-related gene expression levels were quantified with real-time quantitative RT-PCR (D) and western blot (E1) with quantitation (E2).

Techniques: Expressing, Western Blot, Quantitation Assay, Immunofluorescence, Staining, In Vitro, Gene Expression, Quantitative RT-PCR, Labeling, Control